Fully-automated SNPs genotyping system i-densy IS-5310. All-in-one step, Future of SNPs analysis

Principle of SNP typing

A DNA fragment, containing the potential SNP, is amplified through PCR and hybridized with a Qprobe having a complimentary sequence. Utilizing the fact that the dissociation temperature differs according to the conformity of the complimentary sequences, and detecting the fluorescence emitted upon dissociation, SNP is measured.

This system utilizes the QP method (Quenching Probe).

Qprobe is a Probe containing a fluorescently-labeled cytosine base terminal: Qprobe fluorescent emission is quenched by DNA hydridization. Upon dissociation, the fluorescence increases.